Academic Year 2017/2018
- Docente: Pierluigi Strippoli
- Credits: 4
- SSD: BIO/13
- Language: Italian
- Teaching Mode: Traditional lectures
- Campus: Bologna
- Corso: Second cycle degree programme (LM) in Medical Biotechnology (cod. 8859)
Course contents
REGENERATIVE MEDICINE AND GENOMICS (C.I.) A.A. 2017/2018
GENOMICS
Auletta Multimediale, Dipartimento di Medicina Specialistica, Diagnostica e Sperimentale,
Sede Operativa di Istologia, Embriologia e Biologia Applicata, Via Belmeloro 8, 40126 Bologna (BO).
Attending 70% of the lessons/laboratories is required.
Lessons (24 hours)
rational background of common methods in Genomics
1. Human Genomics and Post-Genomics [WED 11 OCTOBER 11, 2017, 14:30-17:00]
2. Bibliography and Genomics databases [WED OCTOBER 18, 2017, 14:30-17:00]
3. Methods for studying nucleic acids (Extraction, Electrophoresis, Enzymes) [THU OCTOBER 19, 2017, 14:30-17:00]
4. Nuclic Acids Hybridization (Southern Blot, Northern Blot, Microarray) [THU OCTOBER 26, 2017, 14:30-17:00]
5. Polymerase Chain Reaction (Kinetics, Parameters, Design) [WED NOVEMBER 08, 2017, 14:30-17:00]
6. cDNA, EST and genomic Libraries [WED NOVEMBER 15, 2017, 14:30-17:00]
7. Nucleic Acids Sequencing and Sequence Analysis [WED 22 NOVEMBER 23, 2017, 15:00-17:30]
8. Computational and System Biology - Trisomy 21 [TUE NOVEMBER 28, 2017, 14:30-17:00]
Laboratory (15 hours)
1. Advanced use of bibliography and genomics databanks [WED OCTOBER 25, 2017, 14:30-17:00]
2. Gene structure - NCBI Gene database [THU NOVEMBER 02, 2017, 14:30-17:00]
3. PCR primer design [THU NOVEMBER 09, 2017, 14:30-17:00]
4. Sequence analysis [THU NOVEMBER 23, 2017, 14:30-17:00]
5. Documentary and discussion [WED JANUARY 10, 2018, 14:30-17:00]
Readings/Bibliography
Fantoni A. et al. Genetica. Piccin, 2009 (Chapter 33: "Human Genome Analysis" - in Italian).
Sito: http://apollo11.isto.unibo.it/
Assessment methods
Short written essay, to be sent as a file via e-mail within the day of the examination.
Teaching tools
Availability of 19 personal computers (iMac) in the "Auletta Multimediale".
Office hours
See the website of Pierluigi Strippoli